Turn-key fluorescence upconversion & Time-Correlated Single Photon Counting Spectroscopy solution featuring high flexibility.

. Fluorescence Upconversion & TCSPC Spectrometer CHIMERA.

Fluorescence Upconversion & Time-Correlated
Single Photon Counting Spectrometer


  • Straightforward operation
  • Automated spectral scanning and upconversion crystal tuning
  • Measure fluorescence dynamics from hundreds of fs to 2 μs in a single instrument


CHIMERA is a time‑resolved fluorescence spectrometer com­bining two techniques of time‑resolved fluorescence. For highest time resolution fluorescence is measured using upconversion technique where the fluorescence light emitted from the sample is mixed in a nonlinear crystal with a fsgating pulse from the laser. The time resolution is then limited by the duration of the gate pulse and is in the range of 250 fs. For fluorescence decay times exceeding 150 ps the instrument can be used in time‑correlated single‑photon counting mode that allows for measuring high accuracy kinetic traces in 200 ps – 2 μs domain.

The combination of two time‑resolved fluorescence techniques enables recording the full decay of fluorescence kinetics at each wavelength; with full data available, spectral calibration of the intensity of kinetic traces taken at different wavelengths is possible, where the integral of time‑resolved data is matched to a steady‑state fluorescence spectrum.

High repetition rate PHAROS laser system allows for measuring fluorescence dynamics while exciting the samples with extre­mely low pulse energies (thereby avoiding exciton annihilation effects in energy transferring systems, or nonlinear carrier recombination in semiconductor/nanoparticle samples).

CHIMERA includes a PC with pre‑installed National Instruments LabView® based measurement automation software that provides the fully controllable, custo­mi­zable and automatic performance of time‑resolved fluorescence measurements. Flexibility of NI LabView allows easy customization of software by the user to tailor it to the needs of specific measurements. Preset or custom delay times, number of averages per transient spectrum and other options are available at a click of the mouse.

In addition to experiment automation software CHIMERA includes the data analysis package CarpetView for inspecting the acquired data and performing global and target analysis, probe dispersion compensation, exponential fitting etc. The software package features an intuitive and user‑friendly interface; it is delivered with a data analysis tutorial that offers seamless transition from the raw data to publi­ca­tion‑quality graphs and model‑based parameter estimation. All the software runs under MS Windows and it is easy to use. Even a novice will become an analysis expert in a matter of days!

Fluorescence Upconversion Mode
Parameter Value
Wavelength range 400 – 1600 nm 1)
Wavelength resolution Limited by the bandwidth of gating pulse, typically around 100 cm-1
Delay range 1.8 ns (3.4 ns, 7.8 ns optional)
Delay step 16.7 fs (33 fs)
Time resolution 1.5 x laser pulse duration (420 fs with standard Pharos laser) 2)
Signal-to-noise 100:1.5, assuming 0.5 s accumulation time per point 3)
Monochromator Newport/Oriel CornerstoneTM 130 USB standart 4)
(See the www.newport.com for detailed specifications)
Crystal rotator XY plane: manual
YZ plane: motorized, 0.01 degree resolution (phase matching angle automatically adjusted by the software while changing wavelengths)
Measurement software Labview – based ChimeraSoft for data collection and experiment automation.
Global and target analysis package for data analysis and presentation. Includes a PC for experiment automation/data analysis
  1. 1)Depending on the gating source, may be achievable with different nonlinear crystals.
  2. 2)Estimated as the FWHM of the upconverted white-light supercontinuum generated in the sample or the derivative of the rise of the upconversion signal.
  3. 3)Estimated as standard deviation of 100 points at 50 ps measured in Rhodamine‑6G dye at 360 nm upconverted wavelength with PHAROS laser running at 150 kHz repetition rate. Not applicable to all samples and configurations.
  4. 4)Other monochromator options possible, such as double subtractive monochromator to ensure high TCSPC time resolution.
TCSPC mode
  • Photochemistry
  • Photobiology
  • Photophysics
  • Material science
  • Semiconductor physics
  • Time-resolved spectroscopy

Products List

Turn-key fluorescence upconversion & Time-Correlated Single Photon Counting Spectroscopy solution featuring high flexibility.

. Fluorescence Upconversion & TCSPC Spectrometer CHIMERA.